Thesis (M.Sc.)--University of Toronto, 1993.
|Series||Canadian theses = Thèses canadiennes|
|The Physical Object|
|Pagination||2 microfiches : negative.|
Based on the high homology of genes coding for guanine nucleotide-binding protein (G protein)-coupled receptors, we have cloned a gene for the Lymnaea stagnalis 5-HT (5HTlym) receptor. Cloning, Characterization, and Expression of a G-Protein-Coupled Receptor from Lymnaea stagnalis and Identiﬁcation of a Leucokinin-Like Peptide, PSFHSWSamide, as Its Endogenous Ligand Kingsley J. A. Cox,1 Cornelis P. Tensen,2 Roel C. Van der Schors,4 Ka Wan Li,4 Harm van Heerikhuizen,3 Erno Vreugdenhil,3 Wijnand P. M. Geraerts,4 and Julian F. Burke1 1Sussex Centre for Neuroscience, . G-protein-coupled receptors as the initial postsynaptic targets. Here we describe the cloning of a neuropeptide receptor from Lymnaea and the isolation of an endogenous ligand. The cloning and characterization of neuropeptide receptors in Lymnaea thus would be very valuable in further elucidating peptidergic pathways. Indirect evidence suggests that these neuropeptides operate via G-protein-coupled mechanisms indicating the presence of G-protein-coupled receptors as the initial postsynaptic targets.
A novel G-protein–coupled receptor (GRL) resembling neuropeptide Y and tachykinin receptors was cloned from the mollusc Lymnaea stagnalis. Application of a peptide extract from the Lymnaea brain to Xenopus oocytes expressing GRL activated a calcium-dependent chloride channel. Using this response as a bioassay, we purified the ligand for GRL, Lymnaea cardioexcitatory peptide . Cloning of the Lymnaea Stagnalis P2X Receptor. P2X CODEHOP PCR primers were designed using the CODEHOP algorithm with input blocks generated from predicted extracellular region amino acid sequences (from the end of transmembrane domain 1 to the start of transmembrane domain 2) of the mammalian P2X and available invertebrate P2X receptors using the BlockS WWW server (Fred . The effects of these neuropeptides are normally mediated via activation of G protein-coupled receptors Alignment of RhoprKR was performed with cloned kinin receptors of invertebrate species W.P.M. Geraerts, J.F. BurkeCloning, characterization, and expression of a g-protein-coupled receptor from Lymnaea stagnalis and identification. An intronless gene encoding a putative G protein-coupled receptor was isolated from the genomic library of barnacle Balanus amphitrite Darwin, with probes obtained from degenerate polymerase chain reaction (PCR) primers used to amplify putative transmembrane regions. The cloned genome DNA specifies an open reading frame of bp encoding amino acids with seven .
A novel G protein-coupled receptor mediating both vasopressin and oxytocin-like functions of Lysconopressin in Lymnea stagnalis. E.R. van Kesteren, C.P. Tensen, A.B. Smit, J. van Minnen, K.S. Kits, W. Meyerhof, We have cloned a receptor, named LSCPR, for vasopressin-related Lys-conopressin in Lymnaea stagnalis. Degenerate oligonucleotide primers, based on conserved regions of the Lymnaea stagnalis 5-HT 1Lym receptor, were used to amplify G protein-coupled biogenic amine receptor sequences from H. trivolvis genomic cDNA, resulting in the cloning of two putative serotonin receptors. The deduced gene products both appear to be G protein-coupled serotonin. found, all encoding putative G protein-coupled receptor fragments. Database searches revealed that one of the cloned cDNAs encoded a protein fragment having a signifi- cantly higher sequence identity with vasopressin and oxy- tocin receptors than with any other G protein-coupled re- ceptor. A novel G-protein–coupled receptor (GRL) resembling neu-ropeptide Y and tachykinin receptors was cloned from the mollusc Lymnaea stagnalis. Application of a peptide extract from the Lymnaea brain to Xenopus oocytes expressing GRL activated a calcium-dependent chloride channel. Us-ing this response as a bioassay, we puriﬁed the ligand for.